Early embryonic development
Differentiation of embryonic germ cells
Mechanisms of mesoderm induction
Isolation and characterization of the mitochondria associated germ cell antigen PG2.
Successful embryonic development depends not only on a properly imprinted cell nucleus but also on many cytoplasmic components and factors provided by the oocyte. A monoclonal antibody raised against the primordial germ cells of rabbits (Viebahn et al. 1998) was found to recognize a mitochondrium-associated antigen in primordial and adult germ cells (Schäfer-Haas and Viebahn 2000; Ricken and Viebahn 2002). This antigen (PG2) is exclusively present in germ cells as wells as in the zygote and the blastomeres of the early embryo. However, the role of this antigen in germ cell differentiation and early development is still unclear. Biochemical characterization will be attempted using lysates of isolated adult germ cells. Additionally cDNA expression libraries in pro- and eukaryotes will be screened to identify and isolate the cDNA coding for the PG2 antigen.
Ricken AM, Viebahn C. Stage-specific expression
of the mitochondrial germ cell epitope PG2 during postnatal differentiation
of rabbit germ cells. Biol Reprod. 2002;67(1):196-203.